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| Haguet, Quentin; Bonnet, Antoine; Berard, Jean-baptiste; Goldberg, Joelle; Joguet, Nicolas; Fleury, Audrey; Thiery, Valerie; Picot, Laurent. |
| Heterocapsa triquetra (Ht), a non-toxic dinophyte, was selected to investigate the presence of pigments inhibiting melanoma cells growth. We developed an innovative microwave-assisted extraction process (MAE), determined the Ht pigment composition using Reverse Phase-High Performance Liquid Chromatography (HPLC) and high-resolution Ultra Performance Liquid Chromatography-Mass Spectrometry E (UPLC-MSE) analysis and assessed their antiproliferative activity in human invasive melanoma cells (A2058) grown in vitro. MAE allowed a fast and efficient extraction of Ht pigments as compared with conventional processes (soaking, sonication). Seventeen pigments and derivatives were unequivocally identified by UPLC-MSᴱ in the Ht extract, among which two were supposed... |
| Tipo: Text |
Palavras-chave: Dinophyte; Heterocapsa; Melanoma; Microalgae; Microwave assisted extraction; Pigment. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00390/50127/50806.pdf |
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| Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Lambert, Christophe; Bideau, Antoine; Bidault, Adeline; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe. |
| The synthetic pathways responsible for the production of the polyunsaturated fatty acids 22:6n-3 and 20:5n-3 were studied in the Dinophyte Alexandrium minutum. The purpose of this work was to follow the progressive incorporation of an isotopic label (13CO2) into 11 fatty acids to better understand the fatty acid synthesis pathways in A. minutum. The Dinophyte growth was monitored for 54 h using high-frequency sampling. A. minutum presented a growth in two phases. A lag phase was observed during the first 30 h of development and had been associated with the probable temporary encystment of Dinophyte cells. An exponential growth phase was then observed after t30. A. minutum rapidly incorporated 13C into 22:6n-3, which ended up being the most 13C-enriched... |
| Tipo: Text |
Palavras-chave: PUFA synthesis; 22:6n-3; DHA; PKS pathway; Dinophyte; Alexandrium minutum; Encystment. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00654/76562/77688.pdf |
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